Anti-nephrolithiatic activity of Garcinia lanceifolia Roxb: An in vitro Study
DOI:
https://doi.org/10.65327/kidneys.v15i1.618Keywords:
G. lanceifolia, urolithiasis, nucleation, aggregation, flavonoidsAbstract
Urolithiasis is a chronic kidney disease characterized by the development of calculi inside the kidneys in a complicated sequence of supersaturation, nucleation, aggregation, crystal development, and retention. The purpose of the current study was to screen the in vitro anti-nephrolithiatic effects of Garcinia lanceifolia Roxb. var. Oxyphylla fruit extract (GLE), which is a potent medicinal plant, against calcium oxalate crystallization. To determine the effect of GLE to prevent calcium oxalate nucleation and aggregation, in vitro studies were carried out. The results demonstrated that GLE may be used as a therapeutic agent for urolithiasis since it significantly (P < 0.001) inhibited the nucleation and aggregation of calcium oxalate crystal. Phytochemical examination indicated GLE has a high amount of total phenolics and flavonoids and also contain reported bioactive compounds like hydroxycitric acid in the fruit of G. lanceifolia. This combined approach may explain the reported anti-nephrolithiatic effects. The findings support the traditional use of G. lanceifolia and the need for additional in vivo studies to determine its therapeutic usefulness and safety.
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Ahmed, S., Hasan, M. M., Khan, H., Mahmood, Z. A., & Patel, S. (2018). The mechanistic insight of polyphenols in calcium oxalate urolithiasis mitigation. Biomedecine & Pharmacotherapie [Biomedicine & Pharmacotherapy], 106, 1292–1299. doi:10.1016/j.biopha.2018.07.080
Gindi, S., Methra, T., Chandu, B. R., Boyina, R., & Dasari, V. (2013). Antiurolithiatic and invitro anti-oxidant activity of leaves of Ageratum conyzoides in rat. World J. Pharm. Pharm. Sci, 2, 636-49.
Ratkalkar, V. N., & Kleinman, J. G. (2011). Mechanisms of stone formation. Clinical Reviews in Bone and Mineral Metabolism, 9(3–4), 187–197. doi:10.1007/s12018-011-9104-8
Sun, X.-Y., Ouyang, J.-M., Wang, F.-X., & Xie, Y.-S. (2015). Formation mechanism of magnesium ammonium phosphate stones: A component analysis of urinary nanocrystallites. Journal of Nanomaterials, 2015(1), 1–9. doi:10.1155/2015/498932
Aggarwal, K. P., Narula, S., Kakkar, M., & Tandon, C. (2013). Nephrolithiasis: molecular mechanism of renal stone formation and the critical role played by modulators. BioMed Research International, 2013, 292953. doi:10.1155/2013/292953
Dika, Ž., Marić, M., & Živko, M. (2025). Treatment of urolithiasis: A comprehensive review. European Medical Journal. Urology, 82–97. doi:10.33590/emjurol/nbza7146
Zumstein, V., Betschart, P., Abt, D., Schmid, H.-P., Panje, C. M., & Putora, P. M. (2018). Surgical management of urolithiasis – a systematic analysis of available guidelines. BMC Urology, 18(1). doi:10.1186/s12894-018-0332-9
Allam, E. A. H., & Sabra, M. S. (2024). Plant-based therapies for urolithiasis: a systematic review of clinical and preclinical studies. International Urology and Nephrology, 56(12), 3687–3718. doi:10.1007/s11255-024-04148-9
Ahmed, S., Hasan, M. M., & Alam Mahmood, Z. (2016). Antiurolithiatic plants: Formulations used in different countries and cultures. Pakistan Journal of Pharmaceutical Sciences, 29(6).
Baruah, S., & Borthakur, S. K. (2012). Studies on morphology and ethnobotany of Six species of GarciniaL.(Clusiaceae) found in the Brahmaputra Valley, Assam, India. J. Nat. Prod. Plant Resour, 2(3), 389-396.
Angami, T., Wangchu, L., Singh, B., Khonglah, L., & Thokchom, A. ( 2021). Garcinia lanceifolia Roxb. (Clusiaceae). In: A. A. Waman, A. A., and Bohra, P. (eds.), Perennial Underutilized Horticultural Species of India. pp181-189.
Bora, N. S., Kakoti, B. B., Bairy, P. S., & Gogoi, B. (2014). Garcinia lanceifolia Roxb; an endemic medicinal plant of assam relieves pain and delays nociceptive response: an assay for its analgesic and anti-inflammatory activity. Int J Pharm Sci Drug Res, 6(3), 216-219.
Gupta, P. C., Kar, A., Sharma, N., Sethi, N., Pandey, P., Saharia, D., & Goswami, N. K. (2019). Pharmacognostic and Physicochemical Studies of Garcinia lanceifolia Roxb. Var. Oxyphylla. Research Journal of Pharmacy and Technology, 12(2), 706. doi:10.5958/0974- 360x.2019.00125.2
Dasgupta, P. (2023).Garcinia lanceifolia Roxb.: An under-utilized endemic horticultural and medicinal crop.Emergent Life Sciences Research,9(2),196-200.dOI: https://doi.org/10.31783/elsr.2023.92196200
Ordonez, A., Gomez, J., Vattuone, M., & Lsla, M. (2006). Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry, 97(3), 452–458. doi:10.1016/j.foodchem.2005.05.024
Zou, Y., Lu, Y., & Wei, D. (2004). Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. Journal of Agricultural and Food Chemistry, 52(16), 5032– 5039. doi:10.1021/jf049571r
Patel, P.K., Patel, M.A., Vyas, B.A., Shah, D.R.,& Gandhi, T.R.(2012). Antiurolithiatic ac tivity of saponin rich fraction from the fruits of Solanum xanthocarpum Schrad. and Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. Journal of Ethnopharmacology, 144, 160–170.http://dx.doi.org/10.1016/j.jep.2012.08.043
Bawari, S., Negi, A.,& Tewari, D. (2018). Antiurolithiatic Activity of Daucus carota:anin vitro study. Pharmacognosy Journal, 10(5), 880–884.
Duan, X., Kong, Z., Mai, X., Lan, Y., Liu, Y., Yang, Z., Zeng, G. (2018). Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Redox Biology, 16, 414–425. doi:10.1016/j.redox.2018.03.019
20. Liu, C.-C., Hsieh, T.-J., Wu, C.-F., Lee, C.-H., Tsai, Y.-C., Huang, T.-Y., Wu, M.-T. (2020). Interrelationship of environmental melamine exposure, biomarkers of oxidative stress and early kidney injury. Journal of Hazardous Materials, 396(122726), 122726. doi:10.1016/j.jhazmat.2020.122726
Khan, M. S., Lari, Q. H., & Khan, M. A. (2016). Anti-urolithiatic unani drugs–A review. World Journal of Pharmaceutical Research, 5(12), 279–294. doi: 10.20959/wjpr201612-7409
Yadav, A., Das, R., Mehta, D. K., & Yatin. (2021). Benefaction of Herbals in the Management of Urolithiasis. Current Traditional Medicine, 7(4), 541-551. doi:10.2174/2215083806999201125122055
Dutta, P. P., Baruah, P., Pathak, B., Barman, D., Devi, D., Deka, K., & Talukdar, N. C. (2023). Quantitative analysis of Garcinol, HCA, HCA lactone, other organic acids, minerals, and antioxidant properties in fruits of eight Garcinia species prevalent in Assam. Annals of Multidisciplinary Research, Innovation and Technology, 2(1), 16-20.
Wesson, J. A., Worcester, E. M., Wiessner, J. H., Mandel, N. S., & Kleinman, J. G. (1998). Control of calcium oxalate crystal structure and cell adherence by urinary macromolecules. Kidney International, 53(4), 952–957. doi:10.1111/j.1523-1755.1998.00839.x
Sheng, X., Ward, M. D., & Wesson, J. A. (2005). Crystal surface adhesion explains the pathological activity of calcium oxalate hydrates in kidney stone formation. Journal of the American Society of Nephrology: JASN, 16(7), 1904–1908. doi:10.1681/ASN.2005040400
Chung, J., Granja, I., Taylor, M. G., Mpourmpakis, G., Asplin, J. R., & Rimer, J. D. (2016). Molecular modifiers reveal a mechanism of pathological crystal growth inhibition. Nature, 536(7617), 446–450. doi:10.1038/nature19062
Doddola, S., Pasupulati, H., Koganti, B., & Prasad, K. V. S. R. G. (2008). Evaluation of Sesbania grandiflora for antiurolithiatic and antioxidant properties. Journal of Natural Medicines, 62(3), 300–307. doi:10.1007/s11418-008-0235-2
Sikarwar, I., Dey, Y. N., Wanjari, M. M., Sharma, A., Gaidhani, S. N., & Jadhav, A. D. (2017). Chenopodium album Linn. leaves prevent ethylene glycol-induced urolithiasis in rats. Journal of Ethnopharmacology, 195, 275–282. doi:10.1016/j.jep.2016.11.031
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